WANG Yu,YOU Jia,CHEN Ao-shuang,et al.The Heterodera glycines Effector Hg16B09 Suppresses Plant Innate Immunity[J].Soybean Science,2021,40(06):759-766.[doi:10.11861/j.issn.1000-9841.2021.06.0759]
大豆胞囊线虫效应蛋白Hg16B09对植物免疫反应的抑制作用
- Title:
- The Heterodera glycines Effector Hg16B09 Suppresses Plant Innate Immunity
- Keywords:
- Soybean; Soybean cyst nematode; Effector; Hg16B09; PAMP-triggered immunity(PTI); Effectors-triggered immunity (ETI); Defense-related genes
- 文献标志码:
- A
- 摘要:
- 为明确大豆胞囊线虫效应蛋白Hg16B09是否对植物免疫反应具有调控作用,本研究借助本氏烟草叶片瞬时表达Hg16B09,利用免疫激发子触发PTI和ETI反应,考察激发子诱导烟草叶片细胞过敏性坏死反应发生、活性氧爆发以及防御相关基因的表达变化情况。结果表明:Hg16B09在烟草细胞中瞬时表达能够显著抑制鞭毛蛋白flg22诱导H2O2的产生及防御基因PTI5、WRKY22-A、 WRKY22-B和ACRE31的表达,其表达量相比对照分别降低了45%、61%、67%和61%。Hg16B09也能抑制由丁香假单胞杆菌Pst DC3000和线虫无毒效应子Rbp-1触发的HR发生,并减少了细胞坏死面积;qRT-PCR分析显示,Hg16B09对Pst DC3000激活的SA途径防御基因PR1a、PR2、WRKY51及PI1上调表达具有显著抑制作用,与对照相比表达量分别降低了67%、71%、48%和81%。以上结果表明Hg16B09对植物的PTI和ETI免疫反应具有双重抑制作用,说明调控寄主免疫防御是Hg16B09促进SCN寄生的一个关键毒性功能。
- Abstract:
- To test whether the effector Hg16B09, identified from the soybean cyst nematode (SCN), Heterodera glycines, plays an important role in manipulating host defense responses, we used Nicotiana benthamiana to transiently express recombinant Hg16B09 plasmid, and different immune elicitors which can trigger defense responses (PTI and ETI). Then, the effects of Hg16B09 on PTI and ETI suppression were investigated by determining the reactive oxygen species (ROS) burst, hypersensitive response (HR), and the expression of defense marker genes. Transient expression of Hg16B09 in Nicotiana benthamiana leaves significantly suppressed the production of hydrogen peroxide and the expression levels of defense-related genes (PTI5, WRKY22-A, WRKY22-B and ACRE31) triggered by flg22. The transcript levels of PTI5, WRKY22-A,WRKY22-B and ACRE31 significantly decreased 45%, 61%, 67% and 61% in N. benthamiana leaves expressing Hg16B09 when compared to that in the control leaves, respectively. Hg16B09 could also inhibit HR induced by Pseudomonas syringae DC 3000 and an avirulent effector Rbp-1, which resulted in the smaller areas of cell death on the N. benthamiana leaves. qRT-PCR analysis further showed that Hg16B09 caused significant down-regulation of defense genes in salicylic acid signaling during Pst DC3000 infection, and the expression levels of PR1a, PR2, WRKY51 and PI1 induced by Pst DC3000 decreased approximately 67%, 71%, 48% and 81% in the leaves expressing Hg16B09 compared to the control leaves, respectively. In conclusion, our data indicated that Hg16B09 acts as PTI and ETI suppressors to perform its toxic biological functions during SCN-host interactions.
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收稿日期:2021-05-28