YANG Hong-yan,HE Qing-yuan,ZHANG Ying-hu,et al.Association Analysis of Seed Traits of Local and Bred Soybean Varieties in Sichuan and Chongqing[J].Soybean Science,2021,40(05):620-627.[doi:10.11861/j.issn.1000-9841.2021.05.0620]
川渝地区地方和育成大豆品种籽粒性状关联分析
- Title:
- Association Analysis of Seed Traits of Local and Bred Soybean Varieties in Sichuan and Chongqing
- Keywords:
- Soybean; Protein content; Oil content; 100-seed weight; Association analysis
- 文献标志码:
- A
- 摘要:
- 为挖掘川渝地区大豆品种籽粒蛋白质含量、油脂含量及百粒重与分子标记的关联位点，以232份川渝地区地方和育成材料构成的自然群体为试验材料，结合分布于大豆20条染色体的135个SSR分子标记检查所有材料的基因型，利用TASSEL 3.0软件中的混合线性模型对大豆籽粒蛋白质含量、油脂含量和百粒重进行关联分析。结果表明：在P<0.05显著水平且贡献率较高的情况下，2个环境中共检测49个关联位点。与蛋白质含量显著关联的位点有16个，贡献率总和为29.78%；与油脂含量相关的位点有19个，贡献率总和为41.94%；与百粒重相关的位点有14个，贡献率总和为13.92%。其中位点Satt554和Satt229与籽粒的蛋白质含量、油脂含量和百粒重性状同时存在关联。
- Abstract:
- In order to explore the association sites of soybean seed protein content，oil content，and 100-seed weight with SSR markers of local and bred soybean varieties in Sichuan and Chongqing region，232 natural populations composed of local and bred materials in Sichuan and Chongqing were used as experimental materials，and 135 SSR molecular markers distributed on 20 chromosomes of soybean were combined to examine the genotypes of all materials.The mixed linear model in TASSEL3.0 software was used to analyze the correlation between soybean seed protein content，oil content and 100-seed weight.The results showed that a total of 49 association sites were detected in the two environments when P<0.05 was significant and the contribution rate was high.There were 16 loci significantly associated with protein content，the total contribution rate was 29.78%，19 loci related to oil content，the total contribution rate was 41.94%，and 14 loci related to 100-seed weight，the total contribution rate was 13.92%.The loci satt554 and satt229 were simultaneously associated with seed protein content，oil content and 100-seed weight.
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备注/Memo
收稿日期：2020-03-04