[1]杨永庆,侯文焕,边全楽,等.河北地区大豆花叶病毒株系的组成与分布[J].大豆科学,2014,33(01):87-90.[doi:10.11861/j.issn.1000-9841.2014.01.0087]
　YANG Yong-qing,HOU Wen-huan,BIAN Quan-le,et al.Composition and Distribution of SMV Strains in Hebei[J].Soybean Science,2014,33(01):87-90.[doi:10.11861/j.issn.1000-9841.2014.01.0087]
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河北地区大豆花叶病毒株系的组成与分布

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第33卷 期数: 2014年01期 页码: 87-90 栏目: 出版日期: 2014-02-25
Title:
Composition and Distribution of SMV Strains in Hebei
文章编号:
1000-9841（2014）01-0087-04
作者:
杨永庆1; 2; 侯文焕1; 边全楽3; 闫 龙1; 张梅申1; 孟小莽4; 刘丽娟1; 林 静1; 智海剑2; 张孟臣1
1.河北省农林科学院 粮油作物研究所/国家大豆改良中心石家庄分中心/农业部黄淮海大豆生物学与遗传育种重点实验室/河北省作物遗传育种实验室，河北 石家庄 050035； 2.南京农业大学 大豆研究所/作物遗传与种质创新国家重点实验室/农业部大豆生物学与遗传育种重点实验室/国家大豆改良中心，江苏 南京 210095； 3.中国农学会，北京 100125； 4.石家庄市农业局，河北 石家庄 050051
Author(s):
YANG Yong-qing1; 2; HOU Wen-huan1; BIAN Quan-le3; YAN Long1; ZHANG Mei-shen1; MENG Xiao-mang4; LIU Li-juan1; LIN Jing1; ZHI Hai-jian2; ZHANG Meng-chen1
1.Institute of Cereal and Oil Crops,Hebei Academy of Agriculture and Forestry Sciences/Shijiazhuang Branch of National Soybean Improvement Center/Huang-Huai-Hai Key Laboratory of Biology and Genetic Improvement of Soybean,Ministry of Agriculture/Hebei Laboratory of Crop Genetics and Breeding,Shijiazhuang 050035,China



关键词:
河北; 大豆花叶病毒; 分离物; 株系; 抗病育种
Keywords:
Hebei; Soybean mosaic virus(SMV); Isolates; Strain; Resistance breeding
分类号:
S565.1
DOI:
10.11861/j.issn.1000-9841.2014.01.0087
文献标志码:
A
摘要:
2012年对河北大豆产区13个县(区)市28个采样点采集的273份大豆花叶病毒（SMV）病样进行了生物纯化，最终共获得67个分离物，经ELISA和RT-PCR检测确认其中56个为SMV分离物。利用全国统一的SMV鉴别体系将这些分离物归为9个株系。与以往的研究结果相比，河北地区SMV株系组成和分布发生一定的变化，其中SC7和SC11仍为流行株系；SC8所占比例有所下降；未检测到SC12、SC13和SC15；发现SC1、SC3和SC18共3个新株系。今后应对SC3株系的发展动态予以密切关注。
Abstract:
A total of 273 virus samples of SMV were collected from 28 locations distributed in 13 counties of Hebei province in 2012.The isolates were biologically purified from collected samples and detected by ELISA and RT-PCR.Then,SMV isolates were classified by the unified national identification system.Finally,67 virus isolates were obtained and 56 virus isolates were identified to be SMV isolates by ELISA and RT-PCR.Base on the differential host responses to these isolates,these SMV isolates were classified into 9 strains.Compared with previous studies,composition and distribution of SMV strains in Hebei had showed some changes,however SC7 and SC11 were still popular strain;the percentage of SC8 strain decreased;SC12,SC13 and SC15 were undetected;three new strains SC1,SC3 and SC18 were first discovered in Hebei,and the development of SC3 strains should be given more attention.

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备注/Memo

基金项目：国家高技术研究发展计划“863计划”（2012AA101106）；现代农业产业技术体系（CARS-004-PS06）；国家“十一五”科技支撑计划（2011BAD35B06-3-6）；河北省科技支撑计划（11220107D）；国家自然科学基金(31171574)。

第一作者简介：杨永庆（1985-），男，博士，主要从事大豆抗病育种研究。E-mail:yyq287346@163.com。
通讯作者：张孟臣（1956-），男，研究员，主要从事作物遗传育种研究。E-mail:mengchenzhang@hotmail.com。智海剑（1957-），男，教授，主要从事大豆抗病遗传育种研究。E-mail:zhj@njau.edu.cn。

更新日期/Last Update: 2014-08-04