FAN Chao,BAI Xi,ZHAO Chao-yue,et al.Investigation and Analysis of the Main Agronomic Traits of Different Transgenic Soybean Lines with GsSAMS Gene[J].Soybean Science,2012,31(02):252-254,258.[doi:10.3969/j.issn.1000-9841.2012.02.018]
转GsSAMS基因大豆主要农艺性状调查与分析
- Title:
- Investigation and Analysis of the Main Agronomic Traits of Different Transgenic Soybean Lines with GsSAMS Gene
- 文章编号:
- 1000-9841（2012）02-0252-03
- Keywords:
- Transgenic soybean; GsSAMS gene; Tolerance to saline and alkaline stress; Agronomic traits
- 分类号:
- S565.1
- 文献标志码:
- A
- 摘要:
- 以非转基因受体绥农28为对照，对前期获得的耐盐碱GsSAMS基因大豆的5个转基因株系SN28-SA-1、SN28-SA-2、SN28-SA-3、SN28-SA-4和SN28-SA-5的T3植株进行了产量性状、品质性状和形态性状的调查与分析。结果表明：在产量性状上，5个转基因大豆株系的单株荚数、单株粒数和百粒重与对照无显著差异；在株高、结荚习性、花色和叶型4个形态性状上，株系SN28-SA-1、SN28-SA-2、SN28-SA-3、SN28-SA-4与对照无显著性差异，但株系SN28-SA-5的株高与对照存在显著性差异（P<0.05），说明同一品种的不同转化株系产生了形态性状变异；在品质性状上，5个转基因株系的蛋白含量高于对照1.42%～2.12%，差异显著，而油份含量差异不显著。因此，转GsSAMS基因大豆的5个转化株系并未在产量性状和品质性状上产生不良变异，同时蛋白含量还有显著提高，具有良好的应用前景。
- Abstract:
- S-adenosyl-L-met-nionine synthetase(SAMS)is a key enzyme in plant metabolize.The syntheses of S-adenosyl-L-methionine(SAM)were catalyzed by SAMS.Transgenic soybean with GsSAMS gene had a greater tolerance to saline and alkaline stress compared with non-transgenic plants.In order to scientific evaluate the variation of the main agronomic traits between transgenic soybean and the control,the yield traits,morphological traits and quality traits of transgenic soybean lines SN28-SA-1,SN28-SA-2,SN28-SA-3,SN28-SA-4 and SN28-SA-5 were investigated and analyzed.There were no obvious differences in yield traits between transgenic soybean and the control.In morphological traits,there were no obvious differences in yield traits between transgenic soybean lines SN28-SA-1，SN28-SA-2，SN28-SA-3,SN28-SA-4 and control,but the plant height of SN28-SA-5 was obvious less than control,which proved different transformation event could produce a variation of morphological traits.In quality traits,the protein contents of all the transgenic soybean lines were higher by 1.42%-2.12% compared with control,while the content of oil was not obviously different between transgenic soybean lines and the control.In conclusion,there was no negetive variation for morphological traits and quality traits in five transgenic soybean lines with GsSAMS gene,meanwhile the protein contents of all the transgenic soybean lines were higher than the contro1,so these transgenic soybean lines has good application prospects.
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备注/Memo
基金项目：转基因生物新品种培育重大专项（2011ZX08004-002）;国家自然科学基金（31171578）；黑龙江省高校科技创新团队建设计划（2011TD055）。