[1]陈晓飞,周伏忠,陈国参,等.纳豆激酶的分离纯化及体外溶栓特性研究[J].大豆科学,2010,29(02):295-298.[doi:10.11861/j.issn.1000-9841.2010.02.0295]
　CHEN Xiao-fei,ZHOU Fu-zhong,CHEN Guo-can,et al.Purification and Thrombolysis Effect of Nattokinase in vitro[J].Soybean Science,2010,29(02):295-298.[doi:10.11861/j.issn.1000-9841.2010.02.0295]
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纳豆激酶的分离纯化及体外溶栓特性研究

《大豆科学》[ISSN:1000-9841/CN:23-1227/S] 卷: 第29卷 期数: 2010年02期 页码: 295-298 栏目: 出版日期: 2010-04-25
Title:
Purification and Thrombolysis Effect of Nattokinase in vitro
文章编号:
1000-9841（2010）02-0295-04
作者:
陈晓飞; 周伏忠; 陈国参; 谢宝恩; 宁 萌
河南省科学院 生物研究所，河南省微生物工程重点实验室，河南 郑州 150008
Author(s):
CHEN Xiao-fei;  ZHOU Fu-zhong;  CHEN Guo-can;  XIE Bao-en;  NING Meng
Biology Institute of Henan Academy of Sciences,Key Laboratory of Microbial Engineering of Henan Province, Zhengzhou 450008，Henan，China
关键词:
纳豆激酶; 分离纯化; 体外溶栓
Keywords:
Nattokinase;  Purification; Thrombolysis in vitro
分类号:
R284.1
DOI:
10.11861/j.issn.1000-9841.2010.02.0295
文献标志码:
A
摘要:
通过硫酸铵分步沉淀和Sephadex G-100 凝胶过滤层析，分离纯化纳豆激酶，并用SDS-PAGE和PAGE电泳检测纯化效果，体外溶栓试验检测纳豆激酶的溶栓特性。SDS-PAGE显示纳豆激酶为单一条带，分子量为33kDa，而PAGE至少有3个具有纤溶活性的区域，表明纳豆激酶可能由几种同工酶组成，体外溶栓结果表明：与蚓激酶相比，纳豆激酶具有较强的的体外溶栓和抗凝作用，并有一定的溶血作用。
Abstract:
Nattokinase was purified by (NH4)2SO4 fractional precipitation and Sephadex G-100 gel filtration from crude Nattokinase liquid and its thrombolytic experiment in vitro were conducted. The purified nattokinase showed a single band on SDS-PAGE with the molecular mass of 33 kDa, while the samples showed three bands on PAGE, which suggested that the Nattokinase was consisted of several isoenzymes. in vitro experiments showed Nattokinase had significant role in thrombolysis and anticoagulation effect，and has some hemolysis effect on rabbit blood cell. Nattokinase maybe have some isoenzymes,which are very important for thrombolysis and anticoagulation.

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备注/Memo

基金项目：河南省省属科研单位社会公益项目（0641180203）。

第一作者简介：张晓飞（1978-）女，硕士，研究方向为微生物发酵工程。E-mail:chenfaye0318@163.com。
通讯作者：周伏忠，研究员。E-mail：zdsyszfz001@163.com。

更新日期/Last Update: 2014-09-13