LI Rui-ning,YAO Xu-yang,ZHAO Tao-di,et al.Identification of Low Temperature Tolerance and Germplasm Screening in Germinating Stage of Vegetable Soybean[J].Soybean Science,2021,40(06):792-798.[doi:10.11861/j.issn.1000-9841.2021.06.0792]
菜用大豆发芽期耐低温性鉴定及种质筛选
- Title:
- Identification of Low Temperature Tolerance and Germplasm Screening in Germinating Stage of Vegetable Soybean
- Keywords:
- Vegetable soybean; Germination period; Low temperature stress; Membership function value; Germplasm screening
- 文献标志码:
- A
- 摘要:
- 大豆种子发芽易受低温条件的影响，造成出苗质量差，进而影响大豆的产量和质量。为比较不同菜用大豆品种在低温胁迫下的耐性，以18份菜用大豆品种为试验材料，在大豆种子萌发期进行15 ℃恒定低温处理，测定其表型指标和抗氧化指标，采用隶属函数法综合评价发芽期不同品种菜用大豆的耐低温特性。结果表明：在15 ℃的低温胁迫下，18份菜用大豆品种的耐低温性差异较大，其中辽鲜、沈鲜15号、优系三和春棚特早发芽势和发芽率均大于95%，具有较高的抗氧化酶活性，且丙二醛含量较低，较高的抗氧化酶活性有助于保护自身免受活性氧的损害，缓解自身的逆境损伤，具有较强的耐低温性；首农8号、沈鲜3号、春丰早早熟60、新福特早、新鲜1号和龙泉神剑的发芽势和发芽指数相对较低，表明在低温环境下其种子发芽慢或者不发芽，萌发受抑制，具有较弱的耐低温性；其他菜用大豆品种的耐低温性均为中等。
- Abstract:
- Soybean seed germination period is easily affected by low temperature conditions, resulting in poor quality of seedling emergence, and then affect the yield and quality of soybean.To compare the resistance of different vegetable soybean varieties under low temperature stress, this study selected 18 vegetable soybean varieties as materials, and carried out 15 ℃ constant low temperature treatment in the germination stage.We measured the phenotypic indexes and antioxidant enzyme activities under different low temperature stress, and then comprehensive evaluated the low temperature tolerance of vegetable soybean of the varieties in the germination stage with membership function method.The results showed that the low temperature tolerance of 18 vegetable soybean varieties was significantly different under the low temperature stress of 15 ℃.The germination energy and germination rate of Liaoxian, Shenxian 15, Youxisan and Chunpengtezao were greater than 95%, with high antioxidant enzyme activity and low MDA content.Higher antioxidant enzyme activity could help protect them from the damage of reactive oxygen species and alleviate the damage of its own stress, so they had a strong low temperature tolerance.The germination energy and germination rate of Shounong 8, Shenxian 3, Chunfengzaozaoshu 60, Xinfutezao, Xinxian 1 and Longquanshenjian were relatively low, indicating that its seeds germinated slowly or did not germinate under low temperature environment, the germination was inhibited, and they had weak low temperature tolerance.The low temperature tolerance of other vegetable soybean varieties were moderate.
参考文献/References:
［1］马丽萍，张彩英，张丽娟，等.菜用大豆的研究进展[J].河北农业科学，2001，5(3):53-57.(Ma L P，Zhang C Y，Zhang L J，et al.Study of progress on vegetable soybean[J].Journal of Hebei Agricultural Sciences，2001，5(3):53-57.)[2]吴早贵.菜用春大豆高产栽培及生态型研究[D].杭州：浙江大学，2004：4-8.(Wu Z G.Study on production，plant physiology and ecotype of spring vegetable soybean[D].Hangzhou：Zhejiang University，2004：4-8.)[3]李照君，田汝美，宫永超，等.基于表型和抗氧化酶活性的大豆萌芽期耐盐性鉴定[J].大豆科学，2020，177(1)：82-89.(Li Z J，Tian R M，Gong Y C，et al.Identification of salt tolerance of soybean in germination stage based on phenotype and antioxidant enzyme activity[J].Soybean Science，2020，177(1)：82-89.)[4]伍祥来，徐志福，李笑，等.菜用大豆高效栽培技术的探讨[J].浙江农业科学，2006(6):621-623.(Wu X L，Xu Z F，Li X，et al.Discussion on efficient cultivation technology of vegetable soybean[J].Journal of Zhejiang Agricultural Sciences，2006(6)：621-623.)[5]李先民，卜朝阳，卢家仕，等.4种处理对杜鹃红山茶种子萌发的影响[J].西部林业科学，2020，49(2)：75-81.(Li X M，Pu C Y，Lu J S，et al.Effect of 4 factors on seed germination of Camellia azalea[J].Journal of West China Forestry Science，2020，49(2)：75-81.)[6]赵云霞，裴红霞，高晶霞，等.27种番茄种质资源种子萌发期耐低温性评价[J].安徽农业科学，2019，47(23):45-48.(Zhao Y X，Pei H X，Gao J X，et al.Evaluation of low temperature resistance of 27 tomato germplasm resources during seed germination[J].Journal of Anhui Agricultural Sciences，2019，47(23)：45-48.)[7]李波，高云鹏，徐艳霞.91份玉米自交系种子耐低温等级的评价[J].种子，2016，35(12):74-77.(Li B，Gao Y P，Xu Y X.Evaluation of low temperature tolerance of 91 maize inbred lines seed[J].Seed,2016，35(12)：74-77.)[8]赵宏光，胡婉莹，郑宇婕，等.不同储存温度和方式对丹参种子萌发的影响[J].种子，2020，39(11):14-18.(Zhao H G，Hu W Y，Zheng Y J，et al.Effects of different storage temperature and methods on seed germination of Salvia miltiorrhiza[J].Seed，2020，39(11)：14-18.)[9]盖志佳，张敬涛，刘婧琦,等.低温胁迫对大豆幼苗形态生理指标及籽粒产量的影响[J].农学学报，2019，9(12)：6-9.(Gai Z J，Zhang J T，Liu J Q，et al.Cold stress affects morphological and physiological indexes of soybean seedling and seed yield[J].Journal of Agriculture，2019，9(12)：6-9.)[10]张影，张跃华，缪天琳，等.低温处理对缬草种子萌发与生理活性的影响[J].中国林副特产，2019(4)：14-16.(Zhang Y，Zhang Y H，Miao T L，et al.Effect of low temperature treatment on the germination and activity of Valeriana officinalis seeds[J].Forest By-Product and Speciality in China，2019(4)：14-16.)[11]夏军，时晓娟，郝先哲，等.低温对不同基因型棉种萌发过程中酶活性及激素含量的影响[J].植物生理学报，2019，55(9)：1291-1305.(Xia J，Shi X J，Hao X Z，et al.Effects of low temperature on enzyme activity and hormone content in germination of different genotypes of cotton seeds[J].Plant Physiology Communications，2019，55(9)：1291-1305.)[12]吕天放，徐田军，刘月娥，等.低温斜坡对不同基因型玉米种子萌发特性的影响[J].玉米科学，2018，26(6)：45-49.(Lyu T F，Xu T J，Liu Y E，et al.Effects of low temperature stress on seed germination of different maize[J].Journal of Maize Sciences，2018，26(6)：45-49.)[13]高茜，徐洪雨，李振松，等.低温斜坡对紫花苜蓿种子萌发的影响[J].草原与草坪，2020，40(4):34-39.(Gao Q，Xu H Y，Li Z S，et al.Effects of chilling stress on seed germination of alfalfa[J].Grassland and Turf，2020，40(4)：34-39.)[14]万茜，刘伟，陈昌友.温度胁迫对豇豆种子萌发生理指标的影响[J].种子，2007，26(10)：32-35.(Wan Q，Liu W，Chen C Y.The Effect of temperature stress on seed germination physiological indices in asparagus bean (Vigna unguiculata L.ssp.Sesquipdalis Verdc.)[J].Seed，2007，26(10)：32-35.)[15]贾祥，陈盼盼，王艳琳，等.4中豆科种子萌发期对低温胁迫的响应[J].高原农业，2020，4(2)：137-142.(Jia X，Chen P P，Wang Y L，et al.Response of four legume species to cold stress during seed germination [J].Journal of Plateau Agriculture，2020，4(2)：137-142.)[16]肖燕子，黄学文，乌仁其其格，等.不同温度处理对呼伦贝尔天然草地3种野生豆科牧草种子萌发的影响[J].畜牧与饲料科学，2020，41(4)：40-44.(Xiao Y Z，Huang X W，Wu R Q Q G，et al.Effects of different temperature treatments on seed germination of three species of wild leguminous forages in Hulun Buir natural grassland[J].Animal Husbandry and Feed Science，2020，41(4)：40-44.)[17]徐来仙，艾训儒，张应团，等.温度及基质对羽扇豆种子发芽的影响[J].湖北农业科学2020，59(17)：75-79.(Xu L X，Ai X R，Zhang Y T，et al.Effect of temperature and matrix on seed germination of Lupinus micranthus [J].Hubei Agricultural Sciences，2020，59(17)：75-79.)[18]郑莉萍，张云峰，蒋洪蔚，等.大豆种质资源芽期耐低温综合评价及筛选[J].大豆科学，2020，39(6)：833-847.(Zheng L P，Zhang Y F，Jiang H W，et al.Comprehensively evaluation and screening on low temperature tolerance of soybean germplasm resources at bud stage[J].Soybean Science，2020，39(6)：833-847.)[19]田鑫，何小玲，顾卫红，等.5份春大豆苗期耐低温性的鉴定及评价[J].大豆科学，2013，32(6)：755-762.(Tian X，He X L，Gu W H，et al.Identification and evaluation of seedling cold tolerance of five spring soybean germplasms[J].Soybean Science，2013，32(6)：755-762.)[20]周玉丽，朱平，胡能兵，等.不同大豆品种发芽期耐旱性评价及耐旱种质筛选[J].大豆科学，2015，34(4)：616-623.(Zhou Y L，Zhu P，Hu N B，et al.Evaluation of drought resistance of different soybean at seed germination stage and the screening of germplasm[J].Soybean Science，2015，34(4)：616-623.)[21]尹燕枰.种子学实验技术[M].北京：中国农业出版社，2008：69-70.(Yin Y P.Experimental techniques of seed science[M].Beijing：Agricultural Press，2008：69-70.)[22]朱莹，孟盺，盖枫.不同温度处理对7种丁香属植物种子萌发特性的影响[J]. 种子，2020，39(12)：32-37. (Zhu Y，Meng X，Ge F，et al. Effects of different temperature treatments on seed germination characteristics of 7 species of Svringa Linn.[J]. Seed，2020，39(12)：32-37.)[23]张丽，贾志国. 低温对不同萌发状态裸燕麦种子生长生理特性的影响[J]. 江苏农业科学，2016，44(6)：161-164. (Zhang L，Jia Z G. Effects of low temperature on growth physiological charac-teristics of naked oat seeds under different germination states[J]. Jiangsu Agricultural Sciences，2016，44(6)：161-164.)[24]崔婷茹，范志强，郭晋杰. 低温胁迫对玉米种子萌发和幼苗活力的影响[J]. 山西农业科学，2019,47(10):1695-1699. (Cui T R，Fan Z Q，Guo J J，et al. Effect of low temperature stress on seed germination and seedling vigor of maize[J]. Journal of Shanxi Agricultural Sciences，2019，47(10)：1695-1699.)[25]乌凤章，王柏臣，刘桂丰,等. 低温胁迫对白桦幼苗生长和生理的影响[J]. 东北林业大学学报，2008，36(9)：8-10. (Wu F Z，Wang B C，Liu G F，et al. Growth and physiological responses of Betula platyphylla seedlings to low temperature stress[J]. Journal of Northeast Forestry University，2008，36(9)：8-10.)[26]杨航宇，刘长仲. 低温胁迫对4种卫矛属植物抗寒生理指标的影响[J]. 草原与草坪，2020，40(2)：94-100. (Yang Y H，Liu C Z. Effects of low temperature stress on physiological indexes of cold resistance of four species in the genus Euonymus[J]. Grassland and Turf，2020，40(2)：94-100.)[27]姜述君，常缨，范文艳,等. 温度逆境锻炼对低温胁迫下番茄幼苗细胞膜脂质过氧化产物及抗氧化酶活性的影响[J]. 中国农学通报，2007，23(10)：444-448. (Jiang S J，Chang Y，Fan W Y，et al. Effects of temperature stress acclimation on peroxidation product of membrane in lipids and anti-oxidative enzyme activities of tomato seedling under cold stress[J]. Chinese Agricultural Science Bulletin，2007，23(10)：444-448.)
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备注/Memo
收稿日期：2021-07-05